{"id":105878,"date":"2023-04-15T11:38:15","date_gmt":"2023-04-15T09:38:15","guid":{"rendered":"https:\/\/www.packaging.si\/what-is-the-difference-between-a-volumetric-pipette-and-a-volumetric-flask\/"},"modified":"2026-10-26T10:35:24","modified_gmt":"2026-10-26T10:35:24","slug":"jaky-je-rozdil-mezi-odmernou-pipetou-a-odmernou-bankou","status":"publish","type":"post","link":"https:\/\/www.packaging.si\/cs\/what-is-the-difference-between-a-volumetric-pipette-and-a-volumetric-flask\/","title":{"rendered":"Jak\u00fd je rozd\u00edl mezi odm\u011brnou pipetou a odm\u011brnou ba\u0148kou?"},"content":{"rendered":"<p>Odm\u011brn\u00e1 pipeta d\u00e1vkuje jeden pevn\u00fd objem kapaliny, zat\u00edmco odm\u011brn\u00e1 ba\u0148ka obsahuje jeden pevn\u00fd objem a pou\u017e\u00edv\u00e1 se k p\u0159\u00edprav\u011b roztok\u016f o p\u0159esn\u00e9m kone\u010dn\u00e9m objemu. Pipeta je kalibrov\u00e1na na v\u00fdtok (TD) a ba\u0148ka na obsah (TC). P\u0159i p\u0159\u00edprav\u011b \u0159ed\u011bn\u00ed se \u010dasto pou\u017e\u00edvaj\u00ed spole\u010dn\u011b.<\/p>\n\n<h2>K \u010demu kter\u00e9 sklo slou\u017e\u00ed<\/h2>\n<p>Odm\u011brn\u00e1 pipeta m\u00e1 dlouh\u00fd tenk\u00fd stonek, st\u0159edn\u00ed ba\u0148ku a jedinou rysku nad ba\u0148kou. Napln\u00edte ji t\u011bsn\u011b nad rysku, nastav\u00edte menisk na \u010d\u00e1ru a nech\u00e1te kapalinu voln\u011b vyt\u00e9ci do jin\u00e9 n\u00e1doby. Hrot se na n\u011bkolik sekund p\u0159ilo\u017e\u00ed k vnit\u0159n\u00ed st\u011bn\u011b a mal\u00e9 mno\u017estv\u00ed zbyl\u00e9 ve hrotu se nevyfukuje.<\/p>\n<p>Odm\u011brn\u00e1 ba\u0148ka m\u00e1 plochou z\u00e1kladnu, \u0161irok\u00e9 hru\u0161kovit\u00e9 t\u011blo a dlouh\u00fd \u00fazk\u00fd kr\u010dek s jednou kruhovou ryskou. P\u0159id\u00e1te rozpu\u0161t\u011bnou l\u00e1tku a trochu rozpou\u0161t\u011bdla, rozpust\u00edte ji a pot\u00e9 opatrn\u011b dopln\u00edte, a\u017e menisk sed\u00ed na rysce. Z\u00e1tka umo\u017e\u0148uje obsah p\u0159evr\u00e1cen\u00edm d\u016fkladn\u011b prom\u00edchat.<\/p>\n\n<h2>Hlavn\u00ed rozd\u00edly<\/h2>\n<table>\n<thead><tr><th>Vlastnost<\/th><th>Odm\u011brn\u00e1 pipeta<\/th><th>Odm\u011brn\u00e1 ba\u0148ka<\/th><\/tr><\/thead>\n<tbody>\n<tr><td>Kalibrace<\/td><td>Na v\u00fdtok (TD)<\/td><td>Na obsah (TC)<\/td><\/tr>\n<tr><td>\u00da\u010del<\/td><td>P\u0159enos p\u0159esn\u00e9ho objemu<\/td><td>P\u0159\u00edprava roztoku o p\u0159esn\u00e9m celkov\u00e9m objemu<\/td><\/tr>\n<tr><td>Typick\u00e9 velikosti<\/td><td>1 a\u017e 100 ml<\/td><td>5 ml a\u017e 2 litry<\/td><\/tr>\n<tr><td>Rysky<\/td><td>Jedna ryska na stonku<\/td><td>Jedna ryska na kr\u010dku<\/td><\/tr>\n<tr><td>Vyprazd\u0148ov\u00e1n\u00ed<\/td><td>Nech\u00e1 se vyt\u00e9ci, hrot p\u0159ilo\u017een ke st\u011bn\u011b<\/td><td>Pro m\u011b\u0159en\u00ed se nevyprazd\u0148uje; obsah se vyl\u00e9v\u00e1 podle pot\u0159eby<\/td><\/tr>\n<tr><td>Skladov\u00e1n\u00ed kapaliny<\/td><td>Ne<\/td><td>Nen\u00ed ur\u010dena pro dlouhodob\u00e9 skladov\u00e1n\u00ed<\/td><\/tr>\n<\/tbody>\n<\/table>\n\n<h2>Pro\u010d z\u00e1le\u017e\u00ed na TD a TC<\/h2>\n<p>Kdy\u017e kapalina st\u00e9k\u00e1 ze skla, z\u016fst\u00e1v\u00e1 na n\u011bm tenk\u00fd film. P\u0159\u00edstroj TD je kalibrov\u00e1n s ohledem na tento film, tak\u017ee objem opou\u0161t\u011bj\u00edc\u00ed pipetu je uveden\u00fd objem. P\u0159\u00edstroj TC obsahuje uveden\u00fd objem, kdy\u017e je napln\u011bn k rysce, ale mno\u017estv\u00ed, kter\u00e9 lze vyl\u00edt, je o n\u011bco men\u0161\u00ed. Proto byste nikdy nem\u011bli pou\u017e\u00edvat ba\u0148ku k odm\u011b\u0159ov\u00e1n\u00ed objemu vylit\u00edm.<\/p>\n<p>Oboj\u00ed je ozna\u010deno teplotou, obvykle 20 stup\u0148\u016f Celsia, proto\u017ee sklo i kapaliny se teplem roztahuj\u00ed. Pou\u017eit\u00ed p\u0159i v\u00fdrazn\u011b odli\u0161n\u00e9 teplot\u011b p\u0159in\u00e1\u0161\u00ed malou, ale re\u00e1lnou chybu.<\/p>\n\n<h2>Spole\u010dn\u00e9 pou\u017eit\u00ed<\/h2>\n<p>B\u011b\u017en\u00fdm postupem je p\u0159\u00edprava z\u0159ed\u011bn\u00e9ho standardu. Odpipetujete p\u0159esn\u00fd alikvot z\u00e1sobn\u00edho roztoku do odm\u011brn\u00e9 ba\u0148ky, p\u0159id\u00e1te rozpou\u0161t\u011bdlo, a\u017e se menisk dotkne rysky, ba\u0148ku uzav\u0159ete z\u00e1tkou a n\u011bkolikr\u00e1t p\u0159evr\u00e1t\u00edte. Pipeta ur\u010duje mno\u017estv\u00ed odebran\u00e9ho z\u00e1sobn\u00edho roztoku a ba\u0148ka kone\u010dn\u00fd objem, tak\u017ee \u0159edic\u00ed faktor je zn\u00e1m p\u0159esn\u011b.<\/p>\n<ul>\n<li>Pipetu p\u0159ed pln\u011bn\u00edm vypl\u00e1chn\u011bte trochou z\u00e1sobn\u00edho roztoku.<\/li>\n<li>Ba\u0148ku z\u00e1sobn\u00edm roztokem nevyplachujte; trocha vody uvnit\u0159 nevad\u00ed, proto\u017ee stejn\u011b p\u0159id\u00e1v\u00e1te rozpou\u0161t\u011bdlo.<\/li>\n<li>Posledn\u00edch n\u011bkolik mililitr\u016f rozpou\u0161t\u011bdla p\u0159idejte kap\u00e1tkem nebo st\u0159i\u010dkou, abyste rysku nep\u0159ekro\u010dili.<\/li>\n<li>M\u00edchejte p\u0159evracen\u00edm; net\u0159epejte bez kontroly rysky.<\/li>\n<\/ul>\n\n<h2>P\u0159esnost<\/h2>\n<p>Sklo t\u0159\u00eddy A m\u00e1 u\u017e\u0161\u00ed tolerance ne\u017e t\u0159\u00edda B. Pipeta 10 ml t\u0159\u00eddy A je obvykle p\u0159esn\u00e1 na asi 0,02 ml a ba\u0148ka 100 ml t\u0159\u00eddy A na asi 0,08 ml. Tyto hodnoty jsou orienta\u010dn\u00ed a p\u0159esn\u00e1 tolerance je vyti\u0161t\u011bna nebo stanovena v p\u0159\u00edslu\u0161n\u00e9 norm\u011b. \u010cistota je z\u00e1sadn\u00ed: tuk na vnit\u0159n\u00edm povrchu zp\u016fsobuje, \u017ee kapky ulp\u00edvaj\u00ed, a zkresluje menisk.<\/p>\n\n<h2>\u010casto kladen\u00e9 ot\u00e1zky<\/h2>\n<h3>Mohu odm\u011brnou ba\u0148ku pou\u017e\u00edt k uchov\u00e1n\u00ed roztoku?<\/h3>\n<p>Je lep\u0161\u00ed ne. Ba\u0148ky jsou ur\u010deny k p\u0159\u00edprav\u011b roztok\u016f a dlouh\u00e9 uchov\u00e1v\u00e1n\u00ed m\u016f\u017ee kapalinu kontaminovat nebo sklo naleptat. Roztok p\u0159elijte do vhodn\u00e9 ozna\u010den\u00e9 lahve.<\/p>\n<h3>Mohu odm\u011brnou ba\u0148ku zah\u0159\u00edvat?<\/h3>\n<p>Vyhn\u011bte se tomu. Zah\u0159\u00edv\u00e1n\u00ed a chlazen\u00ed m\u016f\u017ee trvale zm\u011bnit objem skla, co\u017e zni\u010d\u00ed kalibraci.<\/p>\n<h3>Pro\u010d u odm\u011brn\u00e9 pipety nevyfukuji posledn\u00ed kapku?<\/h3>\n<p>Kalibrace s t\u00edmto mal\u00fdm zbytkem ji\u017e po\u010d\u00edt\u00e1, tak\u017ee jeho vyfouknut\u00ed by vydalo o n\u011bco v\u00edc.<\/p>\n\n<h2>V\u00fdb\u011br kap\u00e1tek pro v\u00e1\u0161 produkt<\/h2>\n<p>Laboratorn\u00ed pipety a ba\u0148ky jsou p\u0159esn\u00e9 n\u00e1stroje. Kap\u00e1tko na lahvi\u010dce produktu funguje jinak: je to sestava sklen\u011bn\u00e9 pipety, gumi\u010dky a uz\u00e1v\u011bru, kter\u00e1 d\u00e1vkuje kapalinu po kapk\u00e1ch a nen\u00ed kalibrov\u00e1na na certifikovan\u00fd objem. Packaging.si sestavuje kap\u00e1tka DIN18 ve Slovinsku a dod\u00e1v\u00e1 odpov\u00eddaj\u00edc\u00ed jantarov\u00e9 sklen\u011bn\u00e9 lahvi\u010dky od 5 do 100 ml.<\/p>\n<ul>\n<li><a href=\"https:\/\/www.packaging.si\/cs\/obchod\/\">Nav\u0161tivte obchod Packaging.si<\/a><\/li>\n<li><a href=\"https:\/\/www.packaging.si\/cs\/kategorie-produktu\/dily-a-komponenty\/\">Pipety, gumi\u010dky a uz\u00e1v\u011bry<\/a><\/li>\n<li><a href=\"https:\/\/www.packaging.si\/cs\/kontaktujte-nas\/\">Vy\u017e\u00e1dat vzorky zdarma<\/a><\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p>A volumetric pipette delivers one fixed volume of liquid, while a volumetric flask contains one fixed volume and is used to prepare solutions of a precise final volume. The pipette is calibrated to deliver (TD), and the flask is calibrated to contain (TC). They are often used together when making dilutions. What each piece of&#8230;<\/p>","protected":false},"author":1,"featured_media":108246,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[237],"tags":[],"class_list":["post-105878","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-glass-pipettes"],"_links":{"self":[{"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/posts\/105878","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/comments?post=105878"}],"version-history":[{"count":1,"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/posts\/105878\/revisions"}],"predecessor-version":[{"id":901995,"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/posts\/105878\/revisions\/901995"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/media\/108246"}],"wp:attachment":[{"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/media?parent=105878"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/categories?post=105878"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.packaging.si\/cs\/wp-json\/wp\/v2\/tags?post=105878"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}